Table 2.
Primers used in this study
| Primer | Sequencea (5′-3′) | Reference or source |
|---|---|---|
| Cloning | ||
| MycBamHI | TAAACCATGGATCCGGAAATTGGTGAAACCG | This study |
| MycXbaI | GAGGATCTAGAAGGGACAGTTTATGTTTAATGGC | This study |
| MBP-BamHIfwd | TCCGGATCCATGGAAATTGGTGAAACCG | This study |
| Rgg-stop-PstI | AAAACTGCAGCCTCAGGACAGTTTATGT TTAATGG | 40 |
| RGG-4 | GGCTATTGACCTTATGCACC | This study |
| VaFwd | CCCCTGATTCTGTGGATAACCGT | This study |
| EMSA and reporter fusions | ||
| speBfwd | GCGGGCATAGTTTTATCAACTGTCATAT | This study |
| speBrev | GCGGGCATAGTTTTATCAACTGTCATA T | This study |
| groEL2fwd | GCTACTCGACGTAACATTGTG | This study |
| groEL2rev | GGAGCCTTCGTACCCAGCAT | This study |
| spd3fwd | GGCGTAGCATTTAAATAAACGGAA | This study |
| spd3rev | GGTGCCTGTAAAATACGAATAAATAAGT | This study |
| pulAfwd | GCGGATCCGATGCCTTTTGTCTCAAACTG | This study |
| pulArev | GCCTCGAGCAACTTGATGTGTTAAGATACTAG | This study |
| 42KDa_BamHIfwd | GCGGATCCTAGTCGGTATCTAGAAGA | This study |
| 42KDa_XhoIrev | GCCTCGAGGAGTTCAGACTTGATTTCTG | This study |
| 1113_BamHIfwd | GCGGATCCCGTATCTGGACTGAGCAAAAC | This study |
| 1113_XhoIrev | GCCTCGAGGCGGTTAATATTTTTGTCAC | This study |
| int_BamHIfwd | GCGGATCCCTACACACTATTACCAC | This study |
| int_XhoIrev | GCCTCGAGTGGTATTCGTGGCGTG | This study |
| pspeB_IRfwd | GCGGATCCATGTCAAGCCTTCCTAGTTGATG | This study |
| pspeB_IRrev | GCCTCGAGTTTTTTTATACCTCTTTCAAAATAAG | This study |
| pspd3_IRfwd | GCGGATCCGTCGGACTAGTCTATGACAAA | This study |
| pspd3_IRrev | GCCTCGAGATCCATGTCCTCCTTTTATTATTTAC | This study |
a
Underlining indicates restriction sites incorporated into the primer.