Table 1.
Plasmids used in this study
| Plasmid | Relevant featuresa | Reference and/or source |
|---|---|---|
| pSD POperondevR | pJFR19 (E. coli-Mycobacterium integrating shuttle vector) containing devR (cloned at NdeI and XbaI sites); DevR is expressed from Rv3134c-devRS operon promoter (−608 to +998 [see reference 8]) cloned in NdeI and BstBI sites, Hygr | 7, 22 |
| pUS POperonT82A | pSD POperondevR harboring T82A mutation, Hygr | This study |
| pAV-DevR | pET28a overexpressing N-terminal His6-tagged WT DevR cloned in BamHI site, Kanr | 21 |
| pUS His6-T82A | pET28a overexpressing DevRT82A cloned in BamHI site, Kanr | This study |
| pSC-DevR | pGEX4T1 overexpressing N-terminal GST-tagged DevR WT cloned in BamHI site, Ampr | 8 |
| pUS GST-T82A | pGEX4T1 overexpressing DevRT82A cloned in BamHI site, Ampr | This study |
| p1738 | pFPV27 (E. coli-Mycobacterium shuttle plasmid with promoter less gfp [see reference 39]) containing Rv1738 promoter, Kanr | 9 |
a
The coordinates of the promoters (in parentheses) are with reference to the transcription start point (TSP) of Rv3134c. Hygr, hygromycin resistance; Kanr, kanamycin resistance.