Table 1.
Plasmid | Relevant featuresa | Reference and/or source |
---|---|---|
pSD POperondevR | pJFR19 (E. coli-Mycobacterium integrating shuttle vector) containing devR (cloned at NdeI and XbaI sites); DevR is expressed from Rv3134c-devRS operon promoter (−608 to +998 [see reference 8]) cloned in NdeI and BstBI sites, Hygr | 7, 22 |
pUS POperonT82A | pSD POperondevR harboring T82A mutation, Hygr | This study |
pAV-DevR | pET28a overexpressing N-terminal His6-tagged WT DevR cloned in BamHI site, Kanr | 21 |
pUS His6-T82A | pET28a overexpressing DevRT82A cloned in BamHI site, Kanr | This study |
pSC-DevR | pGEX4T1 overexpressing N-terminal GST-tagged DevR WT cloned in BamHI site, Ampr | 8 |
pUS GST-T82A | pGEX4T1 overexpressing DevRT82A cloned in BamHI site, Ampr | This study |
p1738 | pFPV27 (E. coli-Mycobacterium shuttle plasmid with promoter less gfp [see reference 39]) containing Rv1738 promoter, Kanr | 9 |
The coordinates of the promoters (in parentheses) are with reference to the transcription start point (TSP) of Rv3134c. Hygr, hygromycin resistance; Kanr, kanamycin resistance.