Table 2.
Strains used in this study
| M. tuberculosis straina | Relevant features | Reference or source |
|---|---|---|
| ΔdevR mutant | 447-bp BalI deletion in M. tuberculosis H37Rv devR gene (deletes DevR amino acid residues from position 40 to 191) | 27 |
| Comp13* | ΔdevR mutant complemented with pSD POperondevR; expresses WT DevR protein | 22 |
| Comp17* | ΔdevR mutant complemented with pUS POperondevR T82A; expresses DevR T82A mutant protein | This study |
| Comp18 | Comp17 electroporated with p1738 (GFP reporter plasmid) | This study |
a
*, M. tuberculosis strains of similar genetic backgrounds that produce DevR T82A (Comp17) or WT protein (Comp13) from a single copy of devR integrated at identical chromosomal locations.