Table 3.
Primers used in this study
| Primer | Sequence (5′→3′)a | Application |
|---|---|---|
| T82A F | TGTCTGATCCTCGCGTCCTACACCTCT | Site-directed mutagenesis (this study) |
| T82A R | AGAGGTGTAGGACGCGAGGATCAGACA | |
| UGSTdevR F | GCCGGATCCATGGTAAAGGTCTTCTTGGTC | Cloning of devR with T82A mutation into pGEX4T1 (this study) |
| GSTdevR R | CCGGGATCCCTATCATGGTCCATCACCGG | |
| RTnarK2 F | CGGTTTGTACGGTGGTTCGGC' | Real-time RT-PCR (this study) |
| RTnarK2 R | TCACGAAGCACGACCATGGCC | |
| RT16S F | ATGACGGCCTTCGGGTTGTAA | Real-time RT-PCR (13) |
| RT16S R | CGGCTGCTGGCACGTAGTTG | |
| RT3134c F | CTGGCTGGGTCGGCCTTAGC | Real-time RT-PCR (13) |
| RT3134c R | TGACCTGGGAGGTTGTCG | |
| RTdevRC F5 | CGAGGATCCCTGTTGTCATGGTCCAT | Real-time RT-PCR (13) |
| RTdevR R | CGCGGCTTGCGTCCGACGTTC | |
| RT devS F | TACTGACCGACCGGGATCGT | Real-time RT-PCR (13) |
| RTdevS R | AGAGCCGCTGGATGACATGG | |
| RT1738 F | CGACGAACACGAAGGATTGA | Real-time RT-PCR (13) |
| RT1738 R | ACACCCACCAATTCCTTTTCC | |
| RT2031c F | CGCACCGAGCAGAAGGA | Real-time RT-PCR (13) |
| RT2031c R | ACCGTGCGAACGAAGGAA | |
| RTtgs1 F | CAGTGATTTGCGTCGCTACAG | Real-time RT-PCR (13) |
| RTtgs1 R | ACATCATTGATGGTGACGTCG | |
| RT3131 F | CGATCAGGCCGATGTCGCCTT | Real-time RT-PCR (13) |
| RT3131 R | TCACCTCCTGGCACCGGCC | |
| 3130F | TGGCTGCCGGGCCTTTCCCAT | DNase I footprinting (10) |
| 3131R | CATGGTCAGCGCCTTCCCCGG | |
| 0571c F | CGGCCGAAGTGAGCCACCACC | DNase I footprinting (11) |
| 0571c R | GCCAAGGACGACGACGGCCTT |
a
BamHI restriction enzyme sites are underlined.