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Densitometric analysis of DNase I footprints for tgs1-Rv3131 target promotera
| Protein (tag, concn) | Protection at P box (ASI) | Protection at S box (ASI) | Ratio of S/P site protection |
|---|---|---|---|
| WT (His, 1.5 μM) | 99 | 98 | 0.99 |
| T82A mutant (His, 1.5 μM) | 60 | 22 | 0.37 |
| WT (GST, 1.5 μM) | 85 | 82 | 0.96 |
| WT (GST, 3.0 μM) | 90 | 88 | 0.98 |
| T82A mutant (GST, 1.5 μM) | 74 | 26 | 0.35 |
| T82A mutant (GST, 3.0 μM) | 80 | 33 | 0.41 |
ASI, arbitrary signal intensity units (average of 3 experiments).
