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Densitometric analysis of DNase I footprints for Rv0571c target promotera
| Protein | GST tag concn (μM) | Protection at P1 box (ASI) | Protection at P2 box (ASI) | Ratio of P2/P1 site protection |
|---|---|---|---|---|
| WT | 1.5 | 90 | 87 | 0.97 |
| 3.0 | 92 | 82 | 0.89 | |
| T82A mutant | 1.5 | 76 | 75 | 0.99 |
| 3.0 | 76 | 79 | 1.04 |
ASI, arbitrary signal intensity units.
