Fig. 3.

(A to C) Rates of DPA release from B. subtilis spores containing only one GR but germinating with multiple germinants. Spores of B. subtilis strains (A, FB87 [lacks the GerB and GerK GRs]; B, FB20 [lacks the GerA GR]; C, PS3665 [has the GerB* GR but lacks the GerA and GerK GRs]) were germinated with various concentrations of l-valine and/or l-asparagine with the GFK components each at 10 mM in germinations with l-asparagine (A and B) or l-valine and/or l-asparagine (C). Rates of DPA release were determined and are given in arbitrary units (au) as described in Materials and Methods. Symbols: ○, l-valine germination; ▵, germination with AGFK or l-asparagine alone; □, experimental curve for germination with l-valine plus AGFK or l-asparagine.