Fig. 6.

(A and B) Commitment and germination of B. subtilis spores germinating with multiple germinants. Spores of B. subtilis strains (A, PS533 [wild type]; B, FB10 [gerB*]) were germinated as described in the text with 0.4 mM l-valine (○ and •), 0.4 mM l-asparagine and each GFK component at 10 mM (▵ and ▴), or 0.4 mM l-valine plus l-asparagine at 0.4 mM and each GFK component at 10 mM (□ and ▪) (A) or with 0.4 mM l-valine (○ and •), 0.1 mM l-asparagine (▵ and ▴), or 0.4 mM l-valine plus 0.1 mM l-asparagine (□ and ▪) (B). During the course of germination, the percentages of spores that had committed (○ ▵, and □) and released DPA (•, ▴, and ▪) were measured as described in Materials and Methods.