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Both cAMP and cGMP inhibit the DNA-binding activity of Vfr T132L T133Y. (A) Protein titration without a ligand and in the presence of 10 μM or 100 μM cAMP. (B) The DNA affinities of 200 nM Vfr T132L T133Y were measured in various concentrations of the ligands cAMP (circles) and cGMP (diamonds).
