Fig. 3.

SDS-PAGE analysis of lipoproteins purified from the lnt-null strain. (A) Pal was purified from DLP79-36 (wt) and LNT6541 (Δlnt), both of which harbored pKM001, and then subjected to 17.5% acrylamide SDS-PAGE (19), followed by staining with CBB. (B) Lpp was expressed from a plasmid, pJY811, in the strains described for panel A, purified, and analyzed by SDS-PAGE (13) and CBB staining. The indicated bands were excised, and the N-terminal amino acid sequences were determined by Edman degradation.