Fig. 4.

Membrane localization of lipoproteins in the lnt-null strain. The wild type (wt) and the lnt-null strain (Δlnt) were transformed with a plasmid encoding LppSR (A), lipoMalE(S) (B), or their derivatives with Asp at position 2 (C and D). Expression of LppSR and LppDR was induced by the addition of arabinose for 30 min. Membranes were separated with a sucrose density gradient into inner and outer membranes. Proteins were detected by immunoblotting using antibodies raised against the respective proteins. LipoMalE was expressed without induction and was detected with anti-maltose-binding protein.