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Ms6 Gp1 homo-oligomerization. Protein extracts from of E. coli BL21(DE3) expressing Gp1 (Gp11-77aa)or Gp1 deletion proteins were cross-linked with different bis(sulfosuccinimidyl)suberate (BS3) concentrations as previously described (1). Proteins were detected by Western blotting with an anti-His6 antibody. Predicted molecular masses (in kDa) of the Gp1 homo-oligomers are indicated to the left of the panels. Oligomerization bands are indicated by arrows.
