Fig. 2.
trans-complementation of nuc expression. (A) Northern blot analysis of nuc in S. aureus Newman, its isogenic ΔyabJ-spoVG mutant SM148, and ΔrsbUVW-sigB mutant IK184 with pBus1 (empty plasmid), pyabJ (PbacA-yabJ), pspoVG (PbacA-spoVG), and pyabJspoVG (PbacA-yabJ-spoVG) after 5 h of growth in LB broth. Transcript masses are indicated. The ethidium bromide-stained 16S rRNA pattern is shown as an indication of the RNA loading. (B) Postulated regulatory pattern of nuc in S. aureus Newman. σB downregulates through activation of SarA and/or other repressors the transcription of nuc. At the same time σB activates SpoVG expression, which in turn counteracts SarA and activates nuc transcription.