. 2011 Sep;193(18):4954–4962. doi: 10.1128/JB.05362-11

Table 1.

Strains and plasmids used in this study

Strain or plasmid	Relevant genotype or phenotype^a	Reference or source
S. aureus strains
Newman	Clinical isolate, ATCC 25904, saeS constitutively active	1, 20
SM148	Newman ΔyabJ-spoVG::erm(B); Em^r	46
MS64	Newman sigB1(Am); Tc^r	47
IK184	Newman ΔrsbUVW-sigB::erm(B); Em^r	32
Coln	Tc^s derivative of S. aureus Col; Mc^r	29
SM165	Coln ΔyabJ-spoVG::erm(B); Em^r	46
BS128	Coln ΔrsbUVW-sigB::erm(B); Em^r	46
Plasmids
pMGS100	Overexpression plasmid containing the bacA promoter of E. faecalis; Cm^r	21
pBus1	E. coli-S. aureus shuttle plasmid with multicloning site from pBlue-script II SK (Stratagene) and the rrnT14 terminator sequence from pLL2443; Tc^r	43
pSTM08	pBus1 with a 2.1-kb PCR fragment covering yabJ, spoVG, and the preceding σ^B-dependent promoter P_yabJ; Tc^r	46
p08-yabJ-His	pSTM08 with a 6-histidine tag added to yabJ; Tc^r	This study
p08-spoVG-His	pSTM08 with a 6-histidine tag added to spoVG; Tc^r	This study
pyabJ	pBus1 containing a bacA promoter-yabJ ORF fusion construct; Tc^r	This study
pyabJ-His	pyabJ with a 6-histidine tag added to yabJ; Tc^r	This study
pspoVG	pBus1 containing a bacA promoter-spoVG ORF fusion construct; Tc^r	This study
pspoVG-His	pspoVG with a 6-histidine tag added to spoVG; Tc^r	This study
pspoVG-Bsu	pBus1 containing the bacA promoter fused to the spoVG ORF of B. subtilis; Tc^r	This study
pyabJspoVG	pBus1 containing a bacA promoter-yabJ-spoVG operon fusion construct; Tc^r	This study

Abbreviations are as follows: Cm^r, chloramphenicol resistant; Em^r, erythromycin resistant; Mc^r, methicillin resistant; Tc^r, tetracycline resistant; Tc^s, tetracycline sensitive.