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Proteins released from B. pertussis cells by osmotic shock treatment. Shock fluids enriched in periplasmic proteins were prepared from cells harvested from iron-replete (+) and iron-depleted (−) SS cultures. A Coomassie blue-stained 12% polyacrylamide gel is shown. Sample loads were normalized to cell numbers based on optical density. Sizes of molecular mass (M) markers are shown in kDa. The arrowheads indicate the position of FbpA.
