Fig. 8.

Secretion profiles of B. pertussis strains Tohama I (A) and UT25 (B) cultured in iron-replete (+Fe) or iron-depleted (−Fe) SS medium. Supernatant fluids were collected at various culture times and the secreted proteins were recovered as described in Materials and Methods. Coomassie blue-stained 12% polyacrylamide gels are shown, with sizes of molecular mass (M) markers given in kDa. Sample loads were normalized to culture cell density. B. bronchiseptica RB50 secreted proteins are shown for comparison. The prominent bands (black arrows) in the RB50 −Fe lanes are Bsp22. Bands (white arrows numbered 1 to 6) were excised from replicate gels and subjected to LC-MS/MS analysis. (C) Protein identities as determined by LC-MS/MS.