Table 1.
Bordetella strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Source |
|---|---|---|
| B. pertussis strains | ||
| Tohama I | Clinical isolate, ca. 1954 | C. Parker |
| UT25 | Clinical isolate, 1975 | C. Parker |
| UT25-90 | Degraded, avirulent phase IV derivative of UT25 | S. Armstrong |
| BP6068 | Clinical isolate, 1997 | J. Miller |
| 165 | Office of Biologics, Food and Drug Administration | C. Manclark via C. Parker |
| 114 | Office of Biologics, Food and Drug Administration | C. Manclark via C. Parker |
| 3779 | Eli Lilly & Co. vaccine strain | C. Parker |
| 10536 | Connaught Laboratories vaccine strain | T. Merkel |
| 188 | Clinical isolate | T. Merkel |
| 461 | Clinical isolate | T. Merkel |
| B. bronchiseptica strains | ||
| RB50 | bvg+ rabbit isolate, wild type | P. Cotter |
| RB53 | bvgS-C3, bvg(Con), Bvg+ phenotypic phase-locked mutant derivative of RB50 | P. Cotter |
| RB54 | ΔbvgAS, Bvg− phenotypic phase-locked mutant derivative of RB50 | P. Cotter |
| Plasmid vectors | ||
| pGEM3Z | 2.7-kb cloning vector, Ampr | Promega |
| pRK415 | 10.5-kb broad-host-range cloning vector, Tetr | N. Keen |
| pRK2013 | 48-kb helper plasmid for mobilization of non-self-transmissible plasmids, Kanr | D. R. Helinski |
| pEG7 | 8.0-kb allelic exchange vector derived from pSS1129, Ampr Gentr | P. Cotter |
| pSS4245 | 8.3-kb allelic exchange vector, I-SceI+, Kanr Strepr | S. Stibitz |
| Recombinant plasmids | ||
| pEG7/fbpABb | 0.8-kb PCR-generated DNA fragment internal to B. bronchiseptica RB50 fbpA gene cloned into pEG7, BamHI-EcoRI primer/adapters, Ampr Gentr | This study |
| pEG7/fbpABp | 0.8-kb PCR-generated DNA fragment internal to B. pertussis Tohama I fbpA gene cloned into pEG7, BamHI-EcoRI primer/adapters, Ampr Gentr | This study |
| pRK/fbpABp | 2.4-kb PCR-generated fbpA+ DNA fragment of B. pertussis Tohama I cloned into pRK415, BamHI-EcoRI primer/adapters, Tetr | This study |
| p3Z/fbpABp | 2.4-kb PCR-generated fbpA+ DNA fragment of B. pertussis Tohama I cloned into pGEM3Z, BamHI-EcoRI primer/adapters, Ampr | This study |
| p3Z/ΔfbpABp | p3Z/fbpABp with 0.7-kb in-frame deletion in fbpA generated by whole-plasmid inverse PCR mutagenesis | This study |
| pSS/ΔfbpABp | 1.7-kb BamHI-EcoRI ΔfbpABp insert fragment of p3Z/ΔfbpABp subcloned into pSS4245, I-SceI+, Kanr, Strepr | This study |
| pRK/fbpABCBp | 6.5-kb fpr fbpABC+ PCR-generated DNA fragment of B. pertussis Tohama I cloned into pRK415, HindIII primer/adapters, Tetr | This study |