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. 2000 May 1;14(9):1048–1057.

Figure 3.

Figure 3

In vivo characterization of the SMRT–TBL1–HDAC3 complex. (a) TBL1 and HDAC3 can each associate with SMRT. Extracts of 293T cells transfected with either Myc–TBL1 or Flag–HDAC3 were immunoprecipitated with anti-myc or anti-flag prior to immunoblot with SMRT monoclonal antibody. All inputs represent 1% of each total immunoprecipitation. (b) HDAC3 interacts with both TBL1 and SMRT in vivo. Extracts of 293T cells transfected with Myc–TBL1 and Flag–HDAC3 were immunoprecipitated with anti-flag prior to immunoblot with SMRT or myc polyclonal antibody. (c) TBL1 associates with histone deacetylase enzymatic activity. 293T cells were transfected with either myc-TBL1 or empty vector alone and subjected to immunoprecipitation with anti-myc followed by assay for deacetylase activity.