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. 2000 May 1;14(9):1048–1057.

Figure 5.

Figure 5

Role of TBL1 in repression. (a) TBL1 potentiates repression by thyroid hormone receptor. A Gal4–TK-luciferase reporter was cotransfected with either Gal4 DBD, Gal4–TR, or Gal4–TR(V230R) and increasing amounts (black ramps representing 0.25–1.5 μg of DNA) of pCMX–HA–TBL1 into HeLa cells. Fold repression was measured as relative to Gal4 DBD alone and the results of duplicate samples are shown. (b) SMRT recruits TBL1 to TR. GST-pulldown assay using GST fusion to TRβ ligand-binding domain with 35S-labeled TBL1 in the presence of control RRL (lanes 2,3) or unlabeled SMRT translated in RRL (lanes 4,5). (c) TBL1 contains an autonomous repression domain. Gal4–TBL1(1–577), Gal4–TBL1(1–211), and Gal4–TBL1(211–577) were transfected with the Gal4–TK-luciferase reporter and fold repression was measured relative to Gal4 DBD alone. (d) GST–TBL1 interacts with histone H3. HeLa Nuclear extract was incubated with either GST alone or GST–TBL1 and interacting histone H3 visualized by immunoblot.