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The degenerate PCR product was amplified from V. anguillarum M93Sm genomic DNA using primer pair Pm301/Pm302. The PCR product was separated and visualized in a 1% agarose gel using a Promega 1-kb DNA ladder as the size standard and was 1.6 kbp long. The PCR product was purified, cleaned, and cloned into pCR2.1 vector and then transformed into the E. coli DH5α strain. The plasmid, purified from the appropriate colony, was sequenced.
