Fig. 8.

Capillary electrophoresis of 6-FAM-labeled DNA fragments b (A) and f (B) from DNase protection assays in the presence (gray traces) and absence (black traces) of HlyU, demonstrating that HlyU binds to specific sequences in fragments b and f of the rtxACHBDE and plp-vah1 intergenic regions, respectively, and protects against DNase I digestion. DNA fragments b and f were prepared and labeled with 6-FAM, reacted with HlyU (0 or 1.88 μg) followed by DNase I, and then analyzed by DNA fragment analysis as described in Materials and Methods. The double black lines show the binding regions. The binding region sequences are shown below the double black line. Underlined bases indicate those that are higher in the presence of HlyU (gray trace) than in its absence (black trace).