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. 2011 Sep;193(18):4779–4789. doi: 10.1128/JB.01033-10

Table 1.

Bacterial strains and plasmids used in this study

Strain or plasmid Genotype and features Source or reference
Strains
    V. anguillarum
        M93Sm Spontaneous Smr mutant of M93 (serotype J-O-1) 3, 4
        S305 Smr Cmr; M93Sm hlyU mutant This study
        S307 Smr Cmr Tetr; M93Sm hlyU complement This study
        S183 Smr Cmr Kanr; M93Sm rtxA vah1 double mutant 10
    E. coli 14
        Sm10 thi thr leu tonA lacY supE recA RP4-2-Tc::Mu::Km (λ pir)
        M15 Nals Strs Rifsthilacara+gal+mtl F−recA+uvr+lon+ (pREP4; Kmr) Qiagen
Plasmids
    pNQ705-1 Cmr; suicide vector with R6K origin 14
    pSUP202 E. coli-V. anguillarum shuttle vector 14
    PCR2.1 Cloning vector Invitrogen
    pQE30UA Expression vector with N-terminal His6 tag Qiagen