Table 2.
Primers used in this study
| Primer | Sequence (5′ to 3′)a | Description |
|---|---|---|
| Pm301 | AGYTAYGARATGYTNGATACGTNAAYTA | Degenerate hlyU F |
| Pm302 | CGTCGYCAGCAYAAYGCTAGCCGTCGYTC | Degenerate hlyU R |
| Pm297 | ACTGAGAGCTCGGTGTTGTTAAAGGCTATGGC | hlyU insertional mutation F |
| Pm298 | ATCGATCTAGAGTATCCACTAACCCATCTCTT | hlyU insertional mutation R |
| R vah1 RT (BF) | GGCTCAACCTCTCCTTGTAACCAA | 5′-RACE vah1 |
| plpF RT | CAGACGACCACCAGTAACCACTAA | 5′-RACE plp |
| Pm112 | TGGTTGTAAGCCGCAGCAC | 5′-RACE rtxH |
| Pm163 | GGGGTATCTGAGTCACATGGATGAATT | 5′-RACE rtxB |
| Primer AP | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV | 5′-RACE anchor primer |
| Pm303 | ATGGAAAAAAATTCCGCTAAAGCA | Entire HlyU protein F |
| Pm304 | CTAGCGGCAGTATAAACCGTGTAA | Entire HlyU protein R |
| Pm305 | CCCGGATCCGCAACTTATCGGTCAGATTGATG | hlyU complementation F |
| Pm306 | CCCGGATCCGATGCGCACTTACATGAAGAAAAC | hlyU complementation R |
a
Restriction sites for SacI (GAGCTC), XbaI (TCTAGA), and EcoRI (GGATCC) are underlined. V=A, C, or G; N=A, C, T, or G; R=A or G; and Y=C or T.