Table 3.
Primers used to amplify DNA probes in the DNase I protection assay
| Intergenic region | Probe no. (length [bp]) | Primer name | Primer sequence | Primer 5′ label | Primer strand |
|---|---|---|---|---|---|
| rtxH-rtxB | 4 (336) | Pm414 | CAGTGGCTCATAAAAGCAGTTGC | 6-FAM | rtxH sense |
| Pm318 | CAGCGGTAAGTAGACTGATA | None | rtxB sense | ||
| 5 (394) | Pm315 | CTCAGACATAAATAAATCACC | None | rtxH sense | |
| Pm415 | CAGCGGTAAGTAGACTGATAAGCAATG | 6-FAM | rtxB sense | ||
| plp-vah1 | 2 (496) | Pm412 | CCGTATTTTCTGCAATCGCCATGG | 6-FAM | plp sense |
| Pm322 | AAAATAAAAGGACATTGGTTTTTTGG | None | vah1 sense | ||
| 3 (468) | Pm327 | GTATTTTCTGCAATCGCCATG | None | plp sense | |
| Pm413 | CACCTTTGTGGCGAATTATTAATAGATCTT | 6-FAM | vah1 sense |