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AMS alkylation to analyze in vivo redox state of TrbB. First lane, oxidized TrbB control (ox); second lane, reduced TrbB control (red); third lane, TrbB in wild-type bacteria (wt); fourth lane, TrbB in dsbD mutant bacteria. TCA-precipitated whole-cell samples were reacted with AMS and visualized by immunoblot staining. TrbB experiences a 1-kDa increase in molecular mass when reduced in vivo.
