Table 1.
Bacterial strains
| Strain | Relevant genotype | Reference or source |
|---|---|---|
| DHB4 | MC1000 [F−araD139 Δ(ara-leu)7697 ΔlacX74pho-510galE15galK16relA1spoT1rpsLthi] phoR68dphoA PvuII ΔmalF3 (F′ lacIqpro) | 7 |
| ER2738 | F′ proA+B+lacIq Δ(lacZ)M15 zzf::Tn10(TetR)/fhuA2glnV Δ(lac-proAB) thi-1 Δ(hsdS-mcrB)5 | NEB |
| JC3272 | JC3051 gal mutant lys mutant | 1 |
| MB69 | DHB4 ΔdsbC | M. Berkmen |
| MB322 | DHB6501 with SM551 | M. Berkmen |
| MB614 | DHB4 with pBAD33-appA | M. Berkmen |
| MB637 | MB69 with pBAD33-appA, pDSW204-dsbC_E.coli | M. Berkmen |
| MB721 | MB69 with pBAD33-appA, pDSW204 | M. Berkmen |
| MB790 | JC3272 with pR27 | M. Berkmen |
| MB794 | MB69 with pBAD33-appA, pDSW204-dsbC-R27 | M. Berkmen |
| MB823 | DHB4 Δara714leu+ | M. Berkmen |
| MB838 | MB69 with pBAD33-appA | M. Berkmen |
| MB872 | MB790 with ΔdsbC-R27::cam | M. Berkmen |
| MB898 | MB790 with ΔdsbC::kan | M. Berkmen |
| MB899 | MB872 with ΔdsbC::kan | M. Berkmen |
| MB900 | MB899 with pDSW204-dsbC_R27 | M. Berkmen |
| MB904 | MB899 with pDSW204-dsbC_E.coli | M. Berkmen |
| MB1171 | MB823 with pBAD33-phoA | M. Berkmen |
| MB1174 | MB823 ΔdsbA::kan | M. Berkmen |
| MB1191 | MB1174 with pBAD33-phoA | M. Berkmen |
| JP114 | ER1821: NEB F−glnV44 e14 mutant (McrA−) rfbD1? relA1? endA1spoT1? thi-1 Δ(mcrC-mrr)114::ISO | J. Bardwell |
| RGP475 | JP114 ΔdsbD::kan | J. Bardwell |
| CH37 | MB899 with pDSW204-trbB | This study |
| CH44 | MB69 with pBAD33-appA, pDSW204-trbB_F | This study |
| CH46 | MB69 with pBAD33-appA, pDSW204-trbB_F C81S/C84S | This study |
| CH50 | MB69 with pBAD33-appA, pDSW204-trbB_F Δ29 | This study |
| CH60 | JP114 with pMER77-trbB_F-FLAG | This study |
| CH61 | RGP475 with pMER77-trbB_F-FLAG | This study |
| CH79 | MB69 ΔdsbD | This study |
| CH80 | CH79 with pBAD33-appA | This study |
| CH81 | CH79 with pBAD-appA, pDSW204-trbB_F | This study |
| CH120 | MB1191 with pDSW204-trbB | This study |