Table 3.
Effect of dsbCE.coli, dsbCR27, and trbB complementation on the mating efficiency of R27 in a ΔdsbCR27 ΔdsbCE.coli double mutant background
| Donor | Genotype | Mating efficiency (no. of transconjugants/donor) | Relative efficiency |
|---|---|---|---|
| MB790 | Wild type | (1.11 ± 0.14) × 10−1 (n=6) | 1 |
| MB872 | ΔdsbCR27 | (1.93 ± 0.20) × 10−2 (n=6) | 0.2 |
| MB898 | ΔdsbCE.coli | (2.88 ± 0.89) × 10−1 (n=6) | 2.6 |
| MB899 | ΔdsbCR27 ΔdsbCE.coli | (6.34 ± 1.05) × 10−6 (n=4) | 0.00006 |
| MB904 | MB899 + dsbCE.coli | (2.90 ± 1.36) × 10−2 (n=3) | 0.26 |
| MB900 | MB899 + dsbCR27 | (6.25 ± 1.41) × 10−3 (n=4) | 0.06 |
| CH37 | MB899 + trbB | (1.76 ± 0.03) × 10−4 (n=8) | 0.002 |
| CH121 | MB899 + trxA | (1.21 ± 0.19) × 10−5 (n=7) | 0.0001 |