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Mutations in the hutPp promoter region. The hutPp DNA sequence from +8 to +31 is shown. The CodY-binding consensus motifs are displayed above the hutPp sequence. The CodY DNA-binding affinities were determined at pH 6.0 in the presence of 32 mM isoleucine and are shown in the rightmost column. The standard errors for the K0.5 values from nonlinear regression analysis of the binding data were less than 10% of each value. ND indicates binding affinities that were not determined.
