Fig. 2.

The 5.5_T7 protein forms a soluble complex when coexpressed with H-NS containing a C-terminal six-histidyl tag. Coomassie-stained SDS-PAGE gels of H-NS_6His expressed from vector pET-21b purified over nickel resin after coexpression with a control pCDF-1b vector (A) or with gp5.5_T7 (B). The lanes include total bacterial protein prior to addition of IPTG (uninduced) and after addition of IPTG (+IPTG) and the eluate after passing cell extracts over nickel column and eluting in the presence of imidazole (Ni²⁺ purified).