Fig. 3.
Diverse 5.5 proteins from various Autographivirinae bind H-NS. (A) Full-length H-NS6His was coexpressed in strains of E. coli BL21(DE3), each containing a plasmid with a gp5.5 homologue as indicated on top. The ability of the two proteins to interact was assessed by SDS-PAGE and Coomassie after copurification using nickel chromatography. Lane labels: M, molecular mass marker; U, uninduced, cell lysate prior to induction with IPTG; I, induced, cell lysate after induction of protein synthesis by IPTG; E, eluate after nickel chromatography. (B) Results of the interaction assay in panel A are shown next to a nearest-neighbor tree of the various gp5.5 homologues. Symbols indicate whether the indicated gp5.5 homologue binds (+), does not bind (−), or displays strongly diminished binding (+/−) to H-NS.