Fig. 5.

Purification of gp5.5_T7 as a soluble and active protein by dissociation from H-NS_60-137. (A) SDS-PAGE of eluted fractions of coexpressed gp5.5_T7 after gel filtration chromatography on a Hiload 16-60 Superdex S200 prep grade column and after ion-exchange chromatography over a HitrapQ fast flow column (Q Sepharose.). (B) Purified gp5.5_T7 was applied to nickel resin either in the absence (left panel) or presence (right panel) of H-NS_6His and association was assessed. The central panel shows H-NS_6His in association with nickel resin in the absence of gp5.5_T7. Lanes: S, supernatant, sample prior to loading onto nickel resin; W, wash fraction; E, eluate after nickel chromatography.