Fig. 6.
Purified 5.5T7 protein disrupts higher-order H-NS/DNA complexes in vitro. A 423-bp fragment of the bglG promoter region was incubated with 500 nM H-NS and increasing concentrations of gp5.5T7 (500 nM, 1,000 nM, 1,500 nM, 2,000 nM, 2,500 nM, and 5,000 nM). The amount of bglG DNA in each reaction was 10 nM. 5.5T7 was incubated with bglG DNA in the absence of H-NS at 500 nM, 1,000 nM, 1,500 nM, 2,000 nM, 2,500 nM, and 5,000 nM. Reactions were then separated on 6% acrylamide gels which were run at 4°C. + and − represent the presence and absence, respectively, of the indicated protein.