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Gel filtration of H-NS, gp5.5T7, and the H-NS/gp5.5T7 complex
| Protein | Elution profilea |
||
|---|---|---|---|
| Concn (μM) | Monomer wt (kDa) | Calculated mass (kDa) | |
| H-NS | 100 | 15.5 | 132 |
| H-NS/gp5.5 | 100 | 163 | |
| gp5.5 | 60 | 11 | 146 |
Calculated mass from Tricorn Superdex 200 10/300 GL that was calibrated immediately prior to use with ribonulease (13.7 kDa), ovalbumin (43 kDa), conalbumin (75 kDa), aldolase (158 kDa), and ferritin (440 kDa), a set of globular proteins with elution profiles that correlate well with mass.
