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. 2011 Sep;31(18):3700–3709. doi: 10.1128/MCB.05448-11

Fig. 5.

Fig. 5.

TSC-22 promotes TGF-β-induced cardiac myofibroblast differentiation. (A) TGF-β upregulates the expression of TSC-22, PAI-1, and α-SMA in primary cardiac fibroblasts from neonatal rats. After treatment with TGF-β1 (100 or 300 pM) for 20 h, the cells were harvested for quantitative RT-PCR. (B) TGF-β enhances the protein levels of TSC-22, PAI-1, and α-SMA in primary rat cardiac fibroblasts. Cells were treated with 100 pM TGF-β1 for 20 h and harvested for immunoblotting. (C) Overexpression of TSC-22 promotes TGF-β-induced cardiac myofibroblast differentiation. Primary rat cardiac fibroblasts were infected with TSC-22 lentivirus. After 24 h, the cells were treated with 100 pM TGF-β1 for 20 h and harvested for quantitative RT-PCR. (D) Knockdown of TSC-22 inhibits TGF-β-induced cardiac myofibroblast differentiation. Primary rat cardiac fibroblasts were transfected with nonspecific or TSC-22 shRNA. At 24 h posttransfection, the cells were treated with TGF-β1 and harvested for quantitative RT-PCR. ColI, collagen I.