Fig. 9.

Y RNA-specific MOs disrupt association between Y RNAs and pre-RC proteins. The four indicated Y RNAs (xY3, xY4, xY5, and zY1) were covalently coupled to adipic acid dihydrazide agarose beads and incubated with HeLa cell extracts in the presence of the indicated antisense MOs (+) or coMO (−). 6B Sepharose beads were used as negative controls (neg). Beads were isolated and washed, and associated proteins were identified by Western blotting. As a reference, 3% of the input cell extract was loaded on the gel. The apparent molecular mass is given for each protein.