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. 2011 Sep;31(18):3773–3789. doi: 10.1128/MCB.05263-11

Fig. 6.

Fig. 6.

LARP6 interacts with vimentin through the La domain. (A) Schematic representation of the constructs used. FS, full-length LARP6. The domains of LARP6 are indicated, with amino acid numbering above. A to I, different deletion mutants of LARP6. All proteins had an HA tag at the N terminus (N-TER). C-TER, C terminus. (B) Interaction of FS LARP6 and deletion mutants with vimentin. (Top) FS LARP6 (lane 1) and the deletion mutants (lanes 2 to 8) were expressed in HEK293 cells. IP was done with antivimentin antibody and Western blotting with anti-HA antibody. (Bottom) Expression of the proteins in the input material. (C) Interaction of the extended La domain with vimentin. A construct containing the intact La domain with additional amino acids at the N terminus (H; lane 3) was analyzed for interaction with vimentin by IP. Lane 1, FS LARP6 as a positive control; lane 2, mutant G as a negative control. (Bottom) Expression of the proteins in the input material. (D) Interaction of the La domain only with vimentin. (Lane 1) Construct I, containing only the La domain, was analyzed for IP with vimentin. (Lane 2) Construct C as a negative control. (Bottom) Expression of the proteins in the input material.