Fig. 2.

Stc2^−/− MEFs display altered Ca²⁺ homeostasis. (A) WT and Stc2^−/− MEFs loaded with 5 μM Fura-2 AM were treated with Tg (300 nM) in the presence of extracellular Ca²⁺ (1.3 mM Ca²⁺ HBSS), followed by perfusion with 0 Ca²⁺ HBSS and the add back of extracellular Ca²⁺ to trigger SOCE. Traces represent the averages from six to seven experiments each for WT and Stc2^−/− MEFs. Average peak Ca²⁺ levels (means ± SEM) after the addition of Tg (B) or extracellular Ca²⁺ (C) for SOCE are shown. (D) WT and Stc2^−/− MEFs were treated with Tg in the absence of extracellular Ca²⁺ before Ca²⁺ add back. Each trace represents averages from five experiments. Graphs represent average peak Ca²⁺ levels (mean ± SEM) after the addition of Tg (E) or extracellular Ca²⁺ (F) for SOCE. **, P < 0.01; ***, P < 0.001 (each by Student's t test).