Fig. 5.

Overexpression of STC2 reduces SOCE. (A) Western blot analysis of STC2 or STC2_KDEL expression in the lysates and conditioned medium of pooled stably transduced MEFs. (B) Control and STC2-overexpressing MEFs loaded with Fura-2 were perfused in 0 Ca²⁺ HBSS before the addition of Tg (300 nM) and Ca²⁺ add back. Traces represent averages from eight experiments. (C) Quantification of [Ca²⁺]_i peaks after Ca²⁺ add back (means ± SEM). (D) Western blot analysis of conditioned medium samples from Stc2^−/− MEFs (KO medium) or Stc2^−/− MEFs overexpressing STC2 (KO+STC2 medium). (E) Fura-2-loaded WT or Stc2^−/− MEFs were incubated in KO or KO+STC2 medium for ∼10 min before Tg was added directly to the media, and [Ca²⁺]_i was monitored over time. Traces represent averages from at least 10 experiments. (F) Quantification of [Ca²⁺]_i peaks after the addition of Tg (means ± SEM). *, P < 0.05; **, P < 0.01; ***, P < 0.001 (each by one-way ANOVA).