Fig. 1.

Schematic representation of the EGFP-based multiple IFN/virus reporter live cell system. HuH-7 cells were treated with recombinant IFN-α2a (100 IU/ml) for 6 h. Total RNA extraction was performed and subjected to whole-genome microarray hybridization and analysis. An IFN-stimulated gene (ISG) cluster was analyzed using bioinformatics by the extraction of the promoters and searching for ISRE/VRE using PromoSer (11) and TFSEARCH (12), respectively. Several variations of ISRE/VRE sequence elements with their context regions were utilized for the construction of EGFP reporters. Cell-based 96-well arrays were assembled for use with various treatments of IFNs and viruses. At the bottom is an image of live cells showing the induction by IFN-α (left) and a mathematical/statistical graph of the quantitative acquisition (right). A reporter with a mutant response element also is shown.