Fig. 5.

PTB, but not Sam68, partially colocalizes with TMEV infection-induced SG. (A) Confocal microscopy images showing coimmunostaining of eIF3 and G3BP or TIA-1 and G3BP in HeLa cells infected for 16 h (10 PFU/cell) with the L^M60V mutant virus. (B) Confocal microscopy images showing coimmunostaining of eIF3 and PTB in HeLa cells infected for 12 h (10 PFU/cell) with wild-type TMEV or with L^Zn and L^M60V mutant viruses. Yellow arrowheads indicate colocalization between PTB and stress granules. Red arrowheads indicate cytoplasmic PTB foci in which no eIF3 was detected. (C) Double fluorescence microscopy images showing representative U3A cells coimmunostained for Sam68 and eIF3, 8 h after infection with 5 PFU/cell of wild-type or L-mutant (L^M60V) TMEV. (D) Detected stress granule markers associated with arsenite-induced SG or TMEV infection-induced SG.