Fig. 6.
Viral RNA is not detected in TMEV-induced SG. (A) In situ hybridization (ISH) to detect positive-stranded viral RNA coupled to immunolabeling of eIF3. HeLa cells were mock infected or infected with wild-type or LM60V TMEV at 10 PFU per cell. After 12 h of infection, cells were fixed and processed for ISH (red) and immunolabeling of eIF3 (green) (confocal microscopy images). (B) Coimmunolabeling of the viral double-stranded RNA (dsRNA; detected with the K1 antibody; red) and eIF3 (green). Experimental conditions were the same as in Fig. 2. (conventional microscopy images).