Fig. 5.

Positively charged-to-alanine scanning mutagenesis and N-terminal deletion of the WhNV B2 protein. (A) Sequence analysis of the B2 ORF and the mutagenesis strategy, with the five alpha-helical regions indicated by “α,” the sites of replacement with alanine indicated by a star, and the N-terminal deletion regions indicated by “▴.” The 11 B2 mutants are shown as R17A, R18A, R24A, R40A, R58A, K6A, K36A, K42A, K75A, K84A, and B2ΔN20. (B) Bacterium-expressed and purified proteins were subjected to 12% SDS-PAGE, followed by Coomassie brilliant blue R250 staining.