Fig. 1.

Purification and identification of LANA binding proteins. (A) A schematic of the procedures used to purify LANA complexes. (B and C) SDS-PAGE of the 8 to 16% gradient gel stained with Coomassie blue. Samples were eluted following immunoprecipitation with rat anti-LANA (α-LANA) antibody from heparin fractions (B) or after additional ion-exchange (MonoQ) purification (C). The isolated protein bands of complexes are denoted with black arrows (LANA, S6, and IgG). Co-IP, coimmunoprecipitation; M, molecular mass markers.