Fig. 3.

GST-CLOCK pulls down ICP4 and ICP27. Purified GST or GST-CLOCK proteins immobilized on beads were reacted with equal amounts of lysates from 2 × 10⁷ HEp-2 cells that were mock infected or exposed to 10 PFU of HSV-1(F) per cell. Immunoblots of the GST or the GST-CLOCK-bound proteins eluted from the glutathione beads with the ICP4 antibody (A) and with the ICP27 antibody (B), as indicated in Materials and Methods. The relevant proteins present in the 1/10 input of the HEp-2 cell lysates used for immune precipitation are shown.