Fig. 4.

CLOCK and BMAL1 exist in a complex that also contains ICP22 in wild-type virus-infected cells. HEp-2 cells (5 × 10⁶) either mock infected or exposed to 10 PFU of HSV-1(F) per cell were harvested at 8 h after infection and lysed. Immunoprecipitations were done with the BMAL1, CLOCK, or control LAMP-1 antibody. Immunoblots were done with the rabbit polyclonal antibody against CLOCK (A) or with the ICP22 antibody (B), as indicated in Materials and Methods. The proteins present in 1/10 of the lysates used for immunoprecipitation are shown.