Fig. 5.

Binding of DNMT3A to Wp. (A) Schematic showing regions of Wp analyzed by X-ChIP. Numbers 1 to 9 represent locations of X-ChIP primers. (B) DNMT1, DNMT3B, or DNMT3A binding to nine regions of the Wp and Cp promoters was measured using X-ChIP. Cp provided a negative control, and Sat2, which has previously been shown to bind DNMT3A and DNMT3B (12), provided a positive control. Nonspecific binding was controlled for by including IgG and no-antibody reactions. Each assay was carried out in duplicate, and results are shown as a percentage of input. Experiments were performed with three GC B-cell-derived LCL, and representative results for one are shown.