Table 1.

Confirmation of array predictions: summary of pyrosequencing results^a

Gene	Cell line	% of genes predicted by array to be hypo- or hypermethylated following EBV infection							% avg change in methylation status across all CpGs examined
		CpG 1	CpG 2	CpG 3	CpG 4	CpG 5	CpG 6	CpG 7
Hypomethylated
CSMD1	GC B	98	89	87	83				−12.5
	LCL	94	80	70	63
SPRY2	GC B	14	12	12	16	13	16		−11
	LCL	2	0	2	7	0	6
MAGEA3	GC B	18	24	37	39				−9.75
	LCL	10	18	29	22
PRDM1	GC B	31	29	30	24	26			−18
	LCL	8	7	14	12	9
ICMT	GC B	44	49	48	88	54			−44
	LCL	11	13	6	26	7
FGFR2	GC B	74	65	72	76	100	86	51	−13.8
	LCL	23	62	64	56	94	78	50
GRB10	GC B	48	91	100	100	80	74		−8.6
	LCL	20	73	99	100	85	65
Hypermethylated
ELL3	GC B	97	62	100	73				8
	LCL	97	94	100	75
TCL6	GC B	31	68	56	100	21			23.2
	LCL	57	95	88	100	52
RBM5	GC B	4	0	4	6	4			2.6
	LCL	11	6	4	6	4
SMAD4	GC B	62	63	50	89	100			6
	LCL	75	70	57	92	100
ID2	GC B	69	69	64	69	61	31		0.7
	LCL	56	90	87	56	52	26

^aThe direction of the methylation change predicted on the array is recorded for each candidate gene along with the percentage of methylation at each CpG site in both LCL and GC B cells. The average percentage of methylation change is also shown.