Fig. 1.

Infectivity, killing and replication of VSV-G/GFP and VSV-rp30a in a diverse panel of human sarcomas. (A and B) Infectivity. Thirteen human sarcoma lines were infected at 5 PFU/cell with either VSV-G/GFP (A) or VSV-rp30a (B). The percentage of cells expressing GFP was assessed at 12 hpi (white bars) and at 36 hpi (black bars). (C) Killing. Sarcomas were mock infected (white bars) or infected at 5 PFU/cell with VSV-G/GFP (gray bars) or VSV-rp30a (black bars). At 36 hpi, cells were incubated with ethidium homodimer (EtHD-1) and the percentage of cells fluorescing red was assessed. (D) Replication. Sarcomas were infected with VSV-G/GFP (gray bars) or VSV-rp30a (black bars) at 1 PFU/cell, washed, and incubated. Supernatants taken at 24 hpi were plaque titered in duplicate on BHK cells. All results were assessed in triplicate. Error bars, SEM. ESFT, Ewing's sarcoma family of tumors; MPNS, malignant peripheral nerve sheath tumor.