Fig. 4.

Synovial sarcoma SW982 resistance to infection. (A) Cell association of VSV-rp30a. Virus-resistant SW982 (white bars) and virus-susceptible S462-TY (black bars) were incubated with 10 PFU/cell of virus under four different conditions (as described in Materials and Methods) designed to measure the efficiency of binding and internalization. After washing, cell-associated genomes from quadruplicate wells were assessed by quantitative RT-PCR normalized to β-actin. Results are normalized to SW982. Error bars, SEM. (B) Resistance to high MOI. Synovial sarcoma SW982 (top) or control sarcoma SJSA-1 (bottom) cells were infected in triplicate by VSV-G/GFP or VSV-rp30a at MOI 0.5, 5, or 50 PFU/cell. The percentage of cells positive for GFP was assessed 12 hpi (gray bars) and 36 hpi (black bars). Error bars, SEM. (C) Resistance to other viruses. SW982, osteosarcoma SJSA-1, or ESFT A673 cells were infected with three recombinant GFP-expressing viruses: VSV, Sindbis virus, or human CMV. Representative micrographs 24 hpi are shown.